| 產品編號 | bs-41358R |
| 英文名稱 | SLC10A2 Rabbit pAb |
| 中文名稱 | 頂膜鈉依賴性膽鹽轉運體蛋白抗體 |
| 別 名 | SLC10A2; Apical sodium dependent bile acid transporter; Apical sodium-dependent bile acid transporter; Ileal sodium dependent bile acid transporter; Ileal sodium-dependent bile acid transporter; Ileal sodium/bile acid cotransporter; ISBT; Na+bile acid cot |
| 研究領域 | 腫瘤 免疫學 信號轉導 通道蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:400-800,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 38 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | Recombinant mouse SLC10A2 protein: 306-348/348 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
SLC10A2 plays a critical role in reabsorption of bile acids from the the small intestine lumen. Passive flow of sodium ions down their concentration gradient is coupled to bile acid movement, resulting in an increase in the concentration of bile acids in the interior of the cell. This action conserves the body's pool of re-circulating bile acid. SLC10A2 also plays a key role in cholesterol metabolism as cholesterol is the precursor molecule in bile acid synthesis mediated by CYP7A and FXR. SWISS: P70172 Gene ID: 20494 Database links: Entrez Gene: 6555 Human Entrez Gene: 20494 Mouse Omim: 601295 Human SwissProt: Q12908 Human SwissProt: P70172 Mouse Unigene: 194783 Human Unigene: 3500 Mouse Unigene: 85891 Rat
|
| 產品圖片 |
Sample:
Lane 1: Human LOVO cell lysates
Lane 2: Human SW480 cell lysates
Lane 3: Human 293T cell lysates
Primary: Anti-SLC10A2 (bs-41358R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kDa
Observed band size: 46 kDa
Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SLC10A2) Polyclonal Antibody, Unconjugated (bs-41358R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Measured by its binding ability in a indirect ELISA. Immobilized Mouse SLC10A2 protein, His Tag (Cat. bs-41358P) at 2 μg/mL (100 μL/well) can bind ?Rabbit Anti-Mouse SLC10A2 Antibody, the EC50 is 179.9 ng/mL.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
