| 產品編號 | bs-8179R |
| 英文名稱 | phospho-Eg5 (Thr926) Rabbit pAb |
| 中文名稱 | 磷酸化驅動蛋白樣蛋白1抗體 |
| 別 名 | Eg5(phospho T926); KIF11(phospho T926); TRIP5(phospho T926); p-Eg5(Thr926); p-KIF11(Thr926); p-TRIP5(Thr926); HKSP; KIF11; kinesin family member 11; Kinesin like protein 1; Kinesin like spindle protein HKSP; KNSL1; Thyroid receptor interacting protein 5; |
| 產品類型 | 磷酸化抗體 |
| 研究領域 | 細胞生物 信號轉導 細胞骨架 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Cow,Chicken,Horse) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 120 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human Eg5 around the phosphorylation site of Thr926: GT(p-T)PQ |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Eg5 is a motor protein that belongs to the kinesin-like family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis. Function: Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays. Subunit: Interacts with the thyroid hormone receptor in the presence of thyroid hormone. Component of a large chromatin remodeling complex, at least composed of MYSM1, PCAF, RBM10 and KIF11/TRIP5. Interacts (via C-terminus) with the kinase NEK6 in both interphase and mitosis. Subcellular Location: Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Post-translational modifications: Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase). Phosphorylated upon DNA damage, probably by ATM or ATR. A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function. Similarity: Belongs to the kinesin-like protein family. BimC subfamily. Contains 1 kinesin-motor domain. SWISS: P52732 Gene ID: 3832 Database links: Entrez Gene: 3832 Human Omim: 148760 Human SwissProt: P52732 Human Unigene: 8878 Human |
| 產品圖片 |
Sample:
HL-60(Human) Cell Lysate at 30 ug
A431(Human) Cell Lysate at 30 ug
U251(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-Eg5 (Thr926) (bs-8179R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 120 kD
Observed band size: 120 kD
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
