| 產(chǎn)品編號 | bs-0017M |
| 英文名稱 | GAL Mouse pAb |
| 中文名稱 | 甘丙肽/神經(jīng)節(jié)肽抗體 |
| 別 名 | GALA_HUMAN; Galanin peptides; Galanin; Galanin message-associated peptide(GMAP); GAL1; GALN; GLNN; galanin and GMAP prepropeptide; ETL8; GMAP; GAL-GMAP; |
| 研究領(lǐng)域 | 心血管 免疫學(xué) 神經(jīng)生物學(xué) 糖尿病 內(nèi)分泌病 |
| 抗體來源 | Mouse |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse,Pig,Sheep,Cow,Chicken,Dog,GuineaPig,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 3/13 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GAL: 33-62/123 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Galanin is small neuropeptide that functions as a cellular messenger within the central and peripheral nervous systems, modulating diverse physiologic functions (Mechenthaler, 2008 [PubMed 18500643]).[supplied by OMIM, Jan 2009] Function: Contracts smooth muscle of the gastrointestinal and genitourinary tract, regulates growth hormone release, modulates insulin release, and may be involved in the control of adrenal secretion. Subcellular Location: Secreted. Similarity: Belongs to the galanin family. SWISS: P22466 Gene ID: 51083 Database links: Entrez Gene: 51083 Human Entrez Gene: 14419 Mouse Omim: 137035 Human SwissProt: P22466 Human SwissProt: P47212 Mouse Unigene: 278959 Human Unigene: 4655 Mouse Unigene: 8929 Rat 神經(jīng)節(jié)肽（Galanin）是由30個氨基酸殘基組成的神經(jīng)肽。最初認為只在相當小的背根神經(jīng)節(jié)細胞群體及脊髓神經(jīng)元中出現(xiàn)，后發(fā)現(xiàn)在消化道及胰腺中也廣泛分布。與感覺的調(diào)整有關(guān)，對痛覺傳遞及脊髓興奮起抑制作用，在很多禽類動物中有表達，是中樞神經(jīng)系統(tǒng)及內(nèi)分泌胰腺的神經(jīng)調(diào)節(jié)肽。 |
| 產(chǎn)品圖片 |
Tissue/cell: rat spinal cord; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Galanin Polyclonal Antibody, Unconjugated(bs-0017M) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0024) and DAB(C-0010) staining
|
| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
