| 產(chǎn)品編號 | bs-1125R |
| 英文名稱 | 5HT1B Receptor Rabbit pAb |
| 中文名稱 | 5-羥色胺受體1B抗體 |
| 別 名 | 5HT1B Receptor; 5 HT 1B; 5 HT 1D beta; 5-HT1BR; 5HT1BR; 5 HT1B; 5 HT1B receptor; 5 HT1DB; 5 hydroxytryptamine(serotonin) receptor 1B; 5 hydroxytryptamine receptor 1B; HTR 1B; HTR1B; HTR1D2; HTR1DB; S12; 5 HT1DB; 5 hydroxytryptamine receptor 1B; 5-HT-1B; 5 |
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Specific References (2) | bs-1125R has been referenced in 2 publications.
[IF=3.18] Liu et al. Fluoxetine attenuates chronic methamphetamine-induced pulmonary arterial remodelling: possible involvement of serotonin transporter and serotonin 1B receptor. (2013) Basic.Clin.Pharmacol.Toxicol. 112:77-82 other ; other.
[IF=2.59] Sun, Tao, et al. "Antihypertensive effect of formononetin through regulating the expressions of eNOS, 5-HT< sub> 2A/1B receptors and ??< sub> 1-adrenoceptors in spontaneously hypertensive rat arteries."European journal of pharmacology (2013).. Rat.
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| 研究領域 | 神經(jīng)生物學 通道蛋白 細胞膜受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 44 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human 5-HTR1B: 121-220/390 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The neurotransmitter serotonin (5-hydroxytryptamine; 5-HT) exerts a wide variety of physiologic functions through a multiplicity of receptors and may be involved in human neuropsychiatric disorders such as anxiety, depression, or migraine. These receptors consist of several main groups subdivided into several distinct subtypes on the basis of their pharmacologic characteristics, coupling to intracellular second messengers, and distribution within the nervous system (Zifa and Fillion, 1992 [PubMed 1359584]). The serotonergic receptors belong to the multigene family of receptors coupled to guanine nucleotide-binding proteins.[supplied by OMIM, Oct 2009]. Function: This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Subcellular Location: Cell membrane; Multi-pass membrane protein. Post-translational modifications: Phosphorylated. Desensitization of the receptor may be mediated by its phosphorylation. Palmitoylated. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P28222 Gene ID: 3351 Database links: Entrez Gene: 3351 Human Omim: 182131 Human SwissProt: P28222 Human Unigene: 123016 Human |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Placenta tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Primary: Anti-5HT1B Receptor (bs-1125R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kDa
Observed band size: 48 kDa
Tissue/cell: rat brain tissue(Snr); 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-5-HTR1B Polyclonal Antibody, Unconjugated(bs-1125R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
